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| Registros recuperados: 66 | |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3). Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf |
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| Aguirre,Carlos; Alvarado,Roberto; Hinrichsen,Patricio. |
| Doce cultivares y líneas de arroz (Oryza sativa L.) desarrollados por el programa de fitomejoramiento del Instituto de Investigaciones Agropecuarias (INIA), Centro Regional de Investigación Quilamapu, fueron caracterizados genéticamente mediante amplificación de fragmentos polimórficos (AFLP). De 21 combinaciones ensayadas, sólo 16 fueron informativas, generando un total de 667 amplicones, 94 de ellos polimórficos (14,4%), con un rango entre 9 y 33% de polimorfismo por combinación. Esto indica que el material manejado por el programa de INIA presenta un bajo nivel de diversidad genética comparado tanto con germoplasma silvestre de la especie como con aquel usado por otros programas de fitomejoramiento de la especie. Los amplicones polimórficos detectados... |
| Tipo: Journal article |
Palavras-chave: Diversidad genética; Fingerprinting; AFLP; Pedigrí; Cultivares chilenos de arroz. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072005000400002 |
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| Santos,Carlos Antônio Fernandes; Oliveira,Viseldo Ribeiro de. |
| O objetivo deste trabalho foi construir um fenograma de trinta indivíduos de seis espécies de Spondias, amostradas em três diferentes locais, baseado em 120 marcadores AFLP das combinações de primers EcoR1/Mse1 para esclarecer as inter-relações entre as espécies, subsidiar a exploração das espécies como porta-enxerto e fornecer informações genômicas dessas espécies. O fenograma UPGMA do coeficiente de Jaccard apresentou boa definição, com valor co-fenético de 0,925 e localização externa de dois "outgroups" das espécies Mangifera indica e Schinopsis brasiliensis da família Anacardeaceae. Todos os indivíduos de S. cytherea, S. tuberosa e S. purpurea foram agrupados dentro de cada espécie, independentemente do local de amostragem, enquanto quatro dos seis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spondias tuberosa; Fenograma; Marcadores moleculares; DNA; AFLP; Genética. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000300028 |
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| Perera,María Francisca; Bertani,Romina Priscila; Arias,Marta Eugenia; Hechavarría,María de la Luz La O; Navarro,María de los Ángeles Zardón; Debes,Mario Alberto; Luque,Ana Catalina; Cuenya,María Inés; Rojas,Ricardo Acevedo; Castagnaro,Atilio Pedro. |
| ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; RDNA; SSR; Infection courts. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200 |
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| Agostinetto,Dirceu; Ulguim,André da Rosa; Vargas,Leandro; Silva,Jessica Dias Gomes da; Langaro,Ana Claudia. |
| Abstract: The objective of this work was to compare wild poinsettia (Euphorbia heterophylla) biotypes as to their morphological characteristics, as well as to determine their genetic similarity in the state of Rio Grande do Sul, Brazil, and to confront the light compensation point of susceptible biotypes and of biotypes with low-resistance level to glyphosate. Besides the morphology analysis, the study included the genetic characterization, by AFLP, of susceptible (GR50= 58.65 g ha-1 a.e.) biotypes and of biotypes with low resistance (GR50= 310.36 g ha-1 a.e.) to glyphosate. The competitive ability of the biotypes was inferred according to their light compensation point. The observed morphological characteristics do not allow to differentiate biotypes as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Euphorbia heterophylla; AFLP; Competitive ability; EPSPs inhibitor herbicide; Light compensation point; Resistance to herbicide. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001100987 |
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| Groenenberg, D.S.J.; Wesselingh, F.P.; Rajagopal, S.; Jansen, J.M.; Bos, M.M.; Velde, G. van der; Gittenberger, E.; Hoeksema, B.W.; Raad, H.; Hummel, H.. |
| Late Quaternary (Eemian) deposits of the Netherlands contain shells that resemble those of living Mytilus galloprovincialis. Similar broad-shelled mytilids also occur in estuaries of the southwestern Netherlands together with slender individuals typical of M. edulis. We sampled living mussels along a depth gradient in the Oosterschelde to a) investigate whether a relation exists between shell shape and depth, b) test if the broadshelled specimens might represent M. galloprovincialis (or a hybrid with M. edulis) and c) assess by inference if the Quaternary specimens might be attributed to M. galloprovincialis as well. In order to do so, we compared genetic (length polymorphism of Me 15/16, COIII sequences and AFLPs) and shellmorphological characteristics... |
| Tipo: Article / Letter to the editor |
Palavras-chave: AFLP; COIII; Me 15/16; Mytilus; Shell morphology; The Netherlands; 42.73. |
| Ano: 2011 |
URL: http://www.repository.naturalis.nl/record/376595 |
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| Borba,Regina da Silva; Loeck,Alci Enimar; Branco,Juliana Severo Castelo; Bonow,Juliane; Oliveira,Antônio Costa de. |
| Este trabalho teve por objetivo estudar a possível ocorrência de heterocariose por meio do pareamento de fungos provenientes de diferentes espécies de formigas cortadeiras coletados em diferentes regiões do Estado do Rio Grande do Sul, utilizando a técnica AFLP. Dez grupos de pareamentos foram realizados, sendo que em dois não ocorreu a fusão das colônias. Após ocorrerem os pareamentos, foram coletados discos de micélio da área de intersecção das colônias e das áreas extremas de pareamento, para a realização da extração do DNA e padronização. Para as reações de PCR, utilizaram-se 21 combinações de primers AFLP, dos quais foram selecionados três que apresentaram maior polimorfismo e consistência. Os dados moleculares foram transformados em matriz binária e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Compatibilidade vegetativa; Marcadores moleculares; Variabilidade; AFLP. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000500002 |
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| Souza,Maria Isabel Ferreira de; Salgueiro,Fabiano; Carnavale-Bottino,Mariana; Félix,Durvalina Benedita; Alves-Ferreira,Marcio; Bittencourt,Juliana Vitoria Messias; Margis,Rogério. |
| Habitat fragmentation and a decrease in population size may lead to a loss in population genetic diversity. For the first time, the reduction in genetic diversity in the northernmost limit of natural occurence (southeastern Brazil) of Araucaria angustifolia in comparison with populations in the main area of the species continuous natural distribution (southern Brazil), was tested. The 673 AFLPs markers revealed a high level of genetic diversity for the species (Ht = 0.27), despite anthropogenic influence throughout the last century, and a decrease of H in isolated populations of southeastern Brazil (H = 0.16), thereby indicating the tendency for higher genetic diversity in remnant populations of continuous forests in southern Brazil, when compared to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araucariaceae; Relict populations; Araucaria forest; Population genetics; AFLP. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300018 |
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| Borba,Regina da Silva; Loeck,Alci Enimar; Branco,Juliana Severo Castelo; Kopp,Maurício Marini; Oliveira,Antônio Costa de. |
| O presente trabalho teve por objetivo verificar o efeito de diferentes períodos de luz ultravioleta sobre a similaridade genética do fungo simbionte de formigas cortadeiras pertencentes às espécies Acromyrmex ambiguus, Acromyrmex crassispinus e Acromyrmex lundi, através da técnica AFLP. Os fungos foram inoculados em meio de cultura Pagnocca e mantidos em câmaras climatizadas à temperatura de 25 + 1°C e escotofase de 24h, por um período de 15 dias. Posteriormente, foram expostos à luz ultravioleta por períodos de: 0, 5, 10, 15, 20, 25 e 30 minutos. Aos 45 dias após o tratamento, foi realizada a extração do DNA e padronização, realizando-se as reações de PCR por meio de 24 combinações de primers AFLP, das quais quatro foram selecionadas por apresentarem... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; Marcadores moleculares; Simbiose. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500001 |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf |
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| Acharya,Laxmikanta; Mukherjee,Arup Kumar; Panda,Pratap Chandra. |
| Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; Cassia; ISSR; Molecular phylogeny; RAPD. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000100026 |
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| Pontaroli,Ana Clara; Camadro,Elsa Lucila. |
| Somaclonal variation in plants regenerated by organogenesis from long-term cultured calluses of two diploid staminate genotypes of Asparagus officinalis cv. Argenteuil was characterized by plant phenotype, ploidy, meiotic behavior, pollen viability, fruit and seed set, and AFLP profiles. Phenotypic deviations from the donors were detected in foliage color, flower size, and cladode and flower morphology. Ploidy changes were observed in 37.8% of the 37 regenerants studied. Meiotic alterations in 12 out of 21 regenerants included laggards, dicentric bridges, micronuclei, restitution nuclei and polyads. Of the 408 AFLP markers screened in 43 regenerants and the donors, 2.94% showed polymorphism. High pollen viability was observed in the 22 regenerants... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; Fertility; Plant morphology; Meiotic behavior; Ploidy. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300015 |
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| Khanlou,Khosro Mehdi; Vandepitte,Katrien; Asl,Leila Kheibarshekan; Bockstaele,Erik Van. |
| Cost reduction in plant breeding and conservation programs depends largely on correctly defining the minimal sample size required for the trustworthy assessment of intra- and inter-cultivar genetic variation. White clover, an important pasture legume, was chosen for studying this aspect. In clonal plants, such as the aforementioned, an appropriate sampling scheme eliminates the redundant analysis of identical genotypes. The aim was to define an optimal sampling strategy, i.e., the minimum sample size and appropriate sampling scheme for white clover cultivars, by using AFLP data (283 loci) from three popular types. A grid-based sampling scheme, with an interplant distance of at least 40 cm, was sufficient to avoid any excess in replicates. Simulations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sampling strategy; White clover; AFLP; Genetic diversity parameters. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200015 |
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| García,Ana M.; Schrauf,Gustavo E.; González,Graciela; Poggio,Lidia; Naranjo,Carlos A.; Dupal,Marck P.; Spangenberg,Germán C.; Forster,John W.. |
| Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B. setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical techniques, revealed variation within this group and indicated that B. setifolius var. brevifolius was closely related to B. setifolius var. pictus, with both taxa being more distantly related to B. setifolius var. setifolius. Cytogenetic analysis confirmed the chromosomal number of B. setifolius var. pictus (2n = 70) and B. setifolius var. setifolius (2n = 28) and showed for the first time that B. setifolius var. brevifolius had 2n = 70. The combination of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; Argentina; Bromus setifolius complex; Cytogenetics; Patagonia; RAPD. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200018 |
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| Shinya,Luciane Tieko; Baccaro,Maria Regina; Moreno,Andrea Micke. |
| Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from swine presenting diarrhea. Analysis of the results showed 29 distinct profiles with discriminatory index equal to 0.97. Partial correlation between the origin of the isolates and groups was drawn, and correlation was possible in only 18.5% of the samples. Characterization of the strains in biotypes (A, B, C, D and E), production of beta-2 toxin and enterotoxin were performed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; Diarrhea; Piglets; Clostridium perfringens. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300034 |
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| Registros recuperados: 66 | |
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